杩愬姩绁炵粡鍏冨瓨娲昏泲鐧界粨鍚堣泲鐧1鎶椾綋
浜у搧鍚嶇О锛杩愬姩绁炵粡鍏冨瓨娲昏泲鐧界粨鍚堣泲鐧1鎶椾綋
浜у搧鍒鍚嶏細 Component of gems 2; GEMI2_HUMAN; Gemin-2; gemin2; SIP 1; SIP-1; SIP1; SIP1 delta; SMN interacting protein 1; SMN interacting protein 1 delta; SMN-interacting protein 1; Survival interacting protein 1; Survival of motor neuron protein interacting protein 1; Survival of motor neuron protein-interacting protein 1. survival of motor neuron protein interacting protein 1; SMN protein interacting protein 1; KIAA0569; SIP 1; SIP1; SMADIP 1; SMADIP1.
鑻辨枃鍚嶇О锛欰nti-Gemin 2
浜у搧缂栧彿锛欻K8988
鎬 鐘讹細Lyophilized or Liquid锛堝喕骞茬矇鎴栨恫浣擄級
娴 搴︼細1mg/1ml
浜у搧瑙勬牸: 0.1ml/0.2ml
鐮旂┒棰嗗煙锛氬績琛绠★紝鍏嶇柅瀛︼紝鏂伴檲浠h阿绛
浜ゅ弶鍙嶅簲锛氫汉锛屽ぇ榧狅紝灏忛紶锛岀姮锛岀尓锛岀緤绛夛紙Human, Mouse, Rat, Dog, Pig, Cow锛
浜у搧搴旂敤锛歐B=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500锛堢煶铚″垏鐗囬渶鍋氭姉鍘熶慨澶嶏級not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
杩愬姩绁炵粡鍏冨瓨娲昏泲鐧界粨鍚堣泲鐧1鎶椾綋鐗圭偣锛
1.璇ユ姉浣撳搧璐ㄩ珮锛岀壒鎰忔у己锛岄珮鎬ц兘锛屼綆鍐呮瘨绱狅紝鎵规¢棿鐨勪竴鑷存хǔ瀹氥傝ユ姉浣撳彈鍒板氬跺浗鍐呴珮鏍¤佸笀鍜屽疄楠屽ょ殑闈掔潗锛屽苟澶氭″洖澶撮噰璐锛屽疄楠岀粨鏋滅ǔ瀹氾紝鍙闈狅紒
2.璇ユ姉浣撻傜敤浜庡氱嶇戠爺瀹為獙锛屽寘鎷珺CIP/NBT,AP锛圓ffinity Purification锛夛紝B/N锛圔locking/Neutralize锛夛紝CD锛圕ell Depletion锛夛紝ELISA(Cap)锛孡ISA(Det)锛孡ISA(Comp)锛孋(Flow Cytometry)锛學B锛圵estern Bolt锛夌瓑銆
杩愬姩绁炵粡鍏冨瓨娲昏泲鐧界粨鍚堣泲鐧1鎶椾綋鐨勪繚瀛樹笌寤鸿锛
鎶椾綋淇濆瓨寰楀綋涓庡惁鐩存帴鍐冲畾浜嗘姉浣撶殑娲绘у拰浣跨敤鏁堟灉銆傚傛灉鎶椾綋淇濆瓨寰楀綋锛屽ぇ閮ㄥ垎鎶椾綋娲绘ч兘鍙浠ョ淮鎸佹暟鏈堢敋鑷虫暟骞淬傝锋寜鐓ц存槑涔︽垨鑰呮姉浣撴爣绛句笂鎺ㄨ崘鐨勪繚瀛樻潯浠舵g‘淇濆瓨鎶椾綋锛佹棤璁烘槸淇濆瓨杩樻槸杩愯緭锛岀粷瀵归伩鍏嶅弽澶嶅喕铻嶆姉浣擄紒
1.鎷垮埌鎶椾綋鍚庤峰姟蹇呭湪1000-3000杞绂诲績1-2鍒嗛挓鍚庡啀鎵撳紑绠$洊杩涜屽垎瑁呭拰淇濆瓨锛侊紙鐢变簬杩愯緭杩囩▼涓鍙嶅嶉犵案锛岀″唴寰閲忔姉浣撳规槗鑱氶泦鍦ㄧ$洊澶勶紝涓鏃︾洿鎺ユ墦寮瀹规槗娴佸け鎶椾綋銆傝锋斁蹇冩姉浣撳嚭鍘傛椂淇濊瘉宸茶呭叆瓒抽噺銆傦級
2.瀵逛簬澶ч儴鍒嗘姉浣擄紝姣旇緝鍚堥傜殑淇濆瓨鏂瑰紡鏄鍒嗚呭悗淇濆瓨鍦-20鈩冨啺绠便傚垎瑁呭彲浠ユ渶澶х▼搴﹂檷浣庡弽澶嶅喕铻嶅规姉浣撴椿鎬х殑鎹熷筹紝鍚屾椂涔熼檷浣庝簡澶氭′粠鍚屼竴绠′腑鍚稿彇鎶椾綋閫犳垚姹℃煋鐨勫彲鑳芥с傚垎瑁呯殑閲忎互涓娆″疄楠岀敤瀹屼负濂斤紝浣嗗缓璁鏈灏戜笉瑕佸皯浜10μL姣忎唤銆傚垎瑁呬綋绉瓒婂皬锛屾姉浣撴祿搴﹀彲鑳戒細鍙楀埌钂稿彂浠ュ強绠″佸惛闄勭殑褰卞搷锛屽悓鏃跺垎瑁呮℃暟瓒婂氱Щ娑插惛澶村惛闄勭殑鎶椾綋涔熻秺澶氾紝鍙鑳戒細璇璁や负鎶椾綋娌℃湁瓒抽噺瑁呭叆銆傦紙鏈濂界敤鏃犺弻鐨勮繘鍙0.2ML绂诲績绠℃潵鍒嗚呮姉浣擄紝鍥戒骇鐨勫皬绠′竴鑸娌℃湁缁忚繃寰堝ソ鐨勭呭寲澶勭悊锛屽规槗鍚搁檮鎶椾綋铔嬬櫧锛屽奖鍝嶆姉浣撴椿鎬с傦級鍒嗚呮椂璇峰皢鏃犺弻鐨勫井閲忕Щ娑插櫒鍚稿ご鍏堟彃鍏ョ″簳閮ㄥ弽澶嶅惞鎵撳嚑娆″悗鍐嶅惛鍑烘姉浣擄紝浠ヤ究鎶椾綋鏈夋晥鎴愬垎涓庢姉浣撲繚鎶ゅ墏鍏呭垎娣峰寑銆傚嶈瀺鍚庣殑鍒嗚呮姉浣撳傛灉涓娆$敤涓嶅畬锛岃峰皢鍓╀綑姣嶆恫淇濆瓨鍦4鈩冨啺绠憋紝閬垮厤鍐嶅喕璧锋潵锛佺粷瀵归伩鍏嶅皢鎶椾綋淇濆瓨鍦ㄨ嚜鍔ㄩ櫎闇滃啺绠辩殑鍐疯棌瀹や腑銆傚敖閲忓皢鎶椾綋淇濆瓨鍦ㄦ墜鍔ㄩ櫎闇滃啺绠遍噷闈锛岃屼笉鏄鍦ㄥ啺绠遍棬涓娿
3.澶ч儴鍒嗘姉浣撴敹鍒板悗4鈩冪煭鏆備繚瀛1-2鍛ㄥ规姉浣撴椿鎬ф槸鍩烘湰娌℃湁褰卞搷鐨勩傚傛灉鎶椾綋寰堝揩锛1-2鍛ㄥ唴锛夊氨鑳戒娇鐢ㄥ畬锛屾帹鑽愬湪4鈩冧繚瀛橈紝閬垮厤鍙嶅嶅喕铻嶅规姉浣撴椿鎬х殑鎹熷炽傚傛灉瑕侀暱鏈熶繚瀛樺垯鍒嗚呭悗-20鈩冧繚瀛樸
4.鐗规畩鎶椾綋鐨勪繚瀛橈細
閰跺伓鑱旀姉浣撲笉搴斿喕瀛橈紝鑰屽簲淇濆瓨浜4鈩冦傚喕铻嶄笉浠呭奖鍝嶆姉浣撶粨鍚堣兘鍔涳紝杩樹細闄嶄綆閰舵椿鎬с傛棤璁哄伓鑱旇嚦鑽у厜鏌撴枡锛岄叾杩樻槸鐢熺墿绱狅紝鍋惰仈鎶椾綋閮藉簲淇濆瓨浜庢繁鑹茶瘯鍓傜摱涓鎴栫敤閲戝睘绠斿寘瑁广傛毚闇蹭簬鍏夌嚎涓灏嗘崯瀹冲伓鑱旂墿鐨勬椿鎬с傜壒鍒鏄鑽у厜鍋惰仈鐗╂槗鍙楀埌鍏夋紓鐧藉奖鍝嶏紝鍦ㄥ疄楠岀殑鎵鏈夐樁娈甸兘搴旈伩鍏夈
杩愬姩绁炵粡鍏冨瓨娲昏泲鐧界粨鍚堣泲鐧1鎶椾綋绛夊叾浠栫儹閿浜у搧濡備笅锛
鍘熺檶鍩哄洜cMAF鎶椾綋
Anti-c-Maf
纾烽吀鍖栧栫汉閿や綋鏋佹牱铔嬬櫧1鎶椾綋
Anti-phospho-Separase (Ser1073)
澶╁啲姘ㄩ吀鍙椾綋鎶椾綋
Anti-NMDA-NR1/NR1/NMDAR1
绁炵粡缁嗚優鑶滅硸铔嬬櫧M6b
Anti-GPM6B
楠ㄨ卞煎洜瀛愭姉浣
Anti-OIF
鐧界粏鑳濬鑲屽姩铔嬬櫧缁撳悎铔嬬櫧鎶椾綋
Anti-LSP1
閰姘ㄩ吀铔嬬櫧婵閰禔4鍙椾綋鎶椾綋
Anti-EphA4 R/Eph receptor A4
7鍙锋煋鑹蹭綋寮鏀鹃槄璇绘29鎶椾綋
Anti-C7ORF29
纭姘ц繕铔嬬櫧缁撳悎铔嬬櫧2鎶椾綋
Anti-TXNIP
鑲屽井绠$礌1鎶椾綋
Anti-MTM1/Myotubularin
鍑濊鍥犲瓙10鎶椾綋
Anti-Factor X
閽欒皟鑺傜礌鎶椾綋锛堥挋璋冭泲鐧/閽欒皟绱狅級
Anti-Calmodulin/CaM I
鐢熺簿缁嗚優鍑嬩骸鐩稿叧鍩哄洜3鎶椾綋
Anti-TSARG3/TSARG1
纾烽吀鍖栨湁涓濆垎瑁傚師鍜屽簲婵娲诲寲鍨嬭泲鐧芥縺閰1鎶椾綋
Anti-Phospho-MSK1 (Ser376)
AF350鏍囪扮殑G铔嬬櫧缁撳悎铔嬬櫧伪16/G伪16鎶椾綋
Anti-G protein alpha 16/AF350